abstract：:The gene for the thermostable pyruvate kinase of Microbispora thermodiastatica IFO 14046, a moderate thermophilic actinomycete, was cloned in Escherichia coli. This gene consists of an open reading frame of 1422 nucleotides and encodes a protein of 474 amino acids with molecular mass of 50,805 Da. The open reading fra...

journal_title：Applied microbiology and biotechnology

authors： Arai A,Masuda S,Matsuyama A,Murakami S,Nakajima M

更新日期：1998-03-01 00:00:00

abstract：:During the process of producing cassava starch from Manihot esculenta roots, large amounts of cyanoglycosides were released, which rapidly decayed to CN- following enzymatic hydrolysis. Depending on the varying cyanoglycoside content of the cassava varieties, the cyanide concentration in the wastewater was as high as ...

journal_title：Applied microbiology and biotechnology

authors： Siller H,Winter J

更新日期：1998-02-01 00:00:00

abstract：:The paper describes the synthesis of the phosphorylcholine-binding miniantibody McPC603scFvDhl x in cell-wall-less L-form strains of Escherichia coli and Proteus mirabilis. Cells of these strains were transformed with the plasmid pACK02scKan, carrying the miniantibody (miniAb) coding sequence under the control of the ...

journal_title：Applied microbiology and biotechnology

authors： Kujau MJ,Hoischen C,Riesenberg D,Gumpert J

更新日期：1998-01-01 00:00:00

abstract：:A Saccharomyces-cerevisiae-based simultaneous saccharification and fermentation (SSF) of lignocellulosic biomass is limited to an operating temperature of about 37 degrees C, and even a small increase in temperature can have a deleterious effect. This points to a need for a more thermotolerant yeast. To this end, S. c...

journal_title：Applied microbiology and biotechnology

authors： Kadam KL,Schmidt SL

更新日期：1997-12-01 00:00:00

abstract：:Potato pulp, one of the agricultural waste products obtained in high quantities during starch production, contains starch, cellulose, hemicelluloses, pectin, proteins, free amino acids and salts. It exhibits physical and physicochemical properties of a typical colloid. It is mainly used, in a dried and pelleted form, ...

journal_title：Applied microbiology and biotechnology

authors： Mayer F,Hillebrandt JO

更新日期：1997-10-01 00:00:00

abstract：:The alpha-amylase of Streptomyces sp. IMD 2679 was subject to catabolite repression. Four different growth rates were achieved when the organism was grown at 40 degrees C and 55 degrees C in the presence and absence of cobalt, with an inverse relationship between alpha-amylase production and growth rate. Highest alpha...

journal_title：Applied microbiology and biotechnology

authors： Mc Mahon HE,Kelly CT,Fogarty WM

更新日期：1997-10-01 00:00:00

abstract：:The influence of low temperature (5-29 degrees C) on the methanogenic activity of non-adapted digested sewage sludge and on temperature/leachate-adapted biomass was assayed by using municipal landfill leachate, intermediates of anaerobic degradation (propionate) and methane precursors (acetate, H2/CO2) as substrates. ...

journal_title：Applied microbiology and biotechnology

authors： Kettunen RH,Rintala JA

更新日期：1997-10-01 00:00:00

abstract：:Removal of tetrachloroethylene (perchloroethylene; C2Cl4) by microbial consortia from two sites with different C2Cl4 exposure histories was examined in a bench-scale anaerobic column bioreactor. It was hypothesized that optimal removal would be observed in the reactor packed with sediments having an extensive exposure...

journal_title：Applied microbiology and biotechnology

authors： Noftsker C,Watwood ME

更新日期：1997-09-01 00:00:00

abstract：:The transport of infectious and biological material is regulated by a number of international organizations. This mini-review has been compiled to increase awareness within the scientific community of problems caused by differences in terminology (such as infectious materials/substances, biological products, diagnosti...

journal_title：Applied microbiology and biotechnology

authors： Lelieveld HL,Bachmayer H,Boon B,Bennett A,Brunius G,Bürki K,Cantley M,Collard JM,Collins CH,Crooy P,Doblhoff-Dier O,Dubakiene R,Economidis I,Elmqvist A,Frontali-Botti C,Havenaar R,Haymerle H,Käppeli O,Leaver G,Lex M

更新日期：1997-08-01 00:00:00

abstract：:Saccharomyces cerevisiae was transformed with the Pichia stipitis CBS 6054 XYL1 and XYL2 genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) respectively. The XYL1 and XYL2 genes were placed under the control of the alcohol dehydrogenase 1 (ADH1) and phosphoglycerate kinase (PGK1) promoters in the yea...

journal_title：Applied microbiology and biotechnology

authors： Walfridsson M,Anderlund M,Bao X,Hahn-Hägerdal B

更新日期：1997-08-01 00:00:00

abstract：:The cultivated and uncultivated bacterial communities of an activated sludge plant were studied. Two samples were taken and a total of 516 bacterial isolates were classified into groups using their whole-cell protein patterns. The distribution of bacteria into protein-pattern groups differed significantly between the ...

journal_title：Applied microbiology and biotechnology

authors： Vainio EJ,Moilanen A,Koivula TT,Bamford DH,Hantula J

更新日期：1997-07-01 00:00:00

abstract：:A fluidized-bed reactor (FBR) was used to enrich an aerobic chlorophenol-degrading microbial culture. Long-term continuous-flow operation with low effluent concentrations selected oligotrophic microorganisms producing good-quality effluent for pentachlorophenol(PCP)-contaminated water. PCP biodegradation kinetics was ...

journal_title：Applied microbiology and biotechnology

authors： Melin ES,Ferguson JF,Puhakka JA

更新日期：1997-06-01 00:00:00

abstract：:Pyranose 2-oxidase (P2O) was purified 43-fold to apparent homogeneity from the basidiomycete Phanerochaete chrysosporium using liquid chromatography on phenyl Sepharose, Mono Q (twice) and phenyl Superose. The native enzyme has a molecular mass of about 250 kDa (based on native PAGE) and is composed of four identical ...

journal_title：Applied microbiology and biotechnology

authors： Artolozaga MJ,Kubátová E,Volc J,Kalisz HM

更新日期：1997-05-01 00:00:00

abstract：:A gene for a synthetic protein-based polymer, G-(VPGVG)119-VPGV, coding for the EG-120mer (elastomer), was cloned into a fungal expression vector to allow constitutive expression of the polymer controlled by the gpdA (glyceraldehyde-3-phosphate dehydrogenase) promoter sequence of Aspergillus nidulans. Stable transform...

journal_title：Applied microbiology and biotechnology

authors： Herzog RW,Singh NK,Urry DW,Daniell H

更新日期：1997-04-01 00:00:00

abstract：:Artificial cold adaptation of a mesophilic protease, subtilisin BPN' was attempted by means of random mutagenesis of its entire gene coupled with screening of cleared-zone-forming colonies on skim-milk plates at a low temperature. Out of sixty clones screened at 10 degrees C, one mutant enzyme (termed M-15) was found ...

journal_title：Applied microbiology and biotechnology

authors： Kano H,Taguchi S,Momose H

更新日期：1997-01-01 00:00:00

abstract：:Xanthomonas campestris pv. campestris, the causal agent of black-rot disease of cruciferous plants, and an important industrial microbe, was able to express the Escherichia coli beta-glucuronidase reporter gene (uidA) when fused to the E. coli lactose operon promoter on a wide-host-range plasmid vector. The gene fusio...

journal_title：Applied microbiology and biotechnology

authors： Soby SD,Daniels MJ

更新日期：1996-12-01 00:00:00

abstract：:The effect of vitamins on the aerobic degradation and dechlorination of 2-chlorophenol and 4-chlorophenol by Pseudomonas pickettii, strain LD1, and 4-chlorobiphenyl by Pseudomonas sp. strain CPE1 was determined. These microorganisms are capable of using the target compounds as the sole carbon and energy source, but do...

journal_title：Applied microbiology and biotechnology

authors： Kafkewitz D,Fava F,Armenante PM

更新日期：1996-11-01 00:00:00

abstract：:The occurrence of killer toxins amongst yeast in Brazilian Riesling Italico grape must was investigated by using the sensitive strain EMBRAPA-26B as a reference strain at 18 degrees C and 28 degrees C. From a total of 85 previously isolated yeasts, 21 strains showed ability to kill the sensitive strain on unbuffered g...

journal_title：Applied microbiology and biotechnology

authors： da Silva GA

更新日期：1996-09-01 00:00:00

abstract：:The Ser-170 residue of a trypsin-like enzyme from Streptomyces fradiae (SFT), which is considered to be the active-site serine, was replaced with alanine by site-directed mutagenesis to improve the affinity chromatography step for a Kazal-type trypsin inhibitor pancreatic secretory trypsin inhibitor (PSTI). The result...

journal_title：Applied microbiology and biotechnology

authors： Katoh T,Kikuchi N,Nagata K,Yoshida N

更新日期：1996-08-01 00:00:00

abstract：:Batch experiments were conducted to assess the biotransformation potential of four hydrocarbon monoterpenes (d-limonene, alpha-pinene, gamma-terpinene, and terpinolene) and four alcohols (arbanol, linalool, plinol, and alpha-terpineol) under aerobic conditions at 23 degrees C. Both forest-soil extract and enriched cul...

journal_title：Applied microbiology and biotechnology

authors： Misra G,Pavlostathis SG,Perdue EM,Araujo R

更新日期：1996-07-01 00:00:00

abstract：:Streptomyces lividans IAF18, obtained by homologous cloning, is capable of over-producing XlnA. To investigate the possibility of the expression of foreign genes, various coding regions of the xylanase A gene (xlnA) were analysed. Expression/secretion vectors were constructed containing the regulatory elements of xlnA...

journal_title：Applied microbiology and biotechnology

authors： Paradis FW,Shareck F,Dupont C,Kluepfel D,Morosoli R

更新日期：1996-06-01 00:00:00

abstract：:Cells of an aerobic three-membered bacterial co-culture, designated as ECO3, capable of cometabolizing and aerobically dechlorinating low-chlorinated biphenyls in the presence of biphenyl, were immobilized on Manville silica beads, on frosted-glass beads and on polyurethane foam cubes in packed-bed bioreactors continu...

journal_title：Applied microbiology and biotechnology

authors： Fava F,Di Gioia D,Marchetti L,Quattroni G

更新日期：1996-05-01 00:00:00

abstract：:Inactivation of D-amino acid oxidase occurred by different mechanisms. The enzyme showed a rapid loss of activity in the presence of micromolar amounts of Cu2+ and Hg2+. It was also sensitive to oxidative inactivation by Fe2+ and H2O2 when both reagents were added in millimolar amounts. When oxidatively inactivated D-...

journal_title：Applied microbiology and biotechnology

authors： Schräder T,Andreesen JR

更新日期：1996-05-01 00:00:00

abstract：:Alkalophilic Bacillus sp. KSM-K16 produced three alkaline proteases, as detected by polyacrylamide gel electrophoresis (PAGE). The major protease, designated M protease, was recently purified to homogeneity and its properties were characterized. In the present study, two minor proteases, designated H protease and N pr...

journal_title：Applied microbiology and biotechnology

authors： Kobayashi T,Hakamada Y,Hitomi J,Koike K,Ito S

更新日期：1996-03-01 00:00:00

abstract：:Lethal effects of pulsed electric fields (PEF) on suspensions of various bacteria, yeast, and spores in buffer solutions and liquid foodstuffs were examined. Living-cell counts of vegetative cell types were reduced by PEF treatment by up to more than four orders of magnitude (> 99.99%). On the other hand, endo- and as...

journal_title：Applied microbiology and biotechnology

authors： Grahl T,Märkl H

更新日期：1996-03-01 00:00:00

abstract：:A study was conducted to optimize the biodegradation in soil slurries of phenanthrene initially dissolved in nonaqueous-phase liquids (NAPLs). The slow rate of degradation of phenanthrene in dibutyl phthalate was increased by addition of phenanthrene-degrading microorganisms to soil slurries containing the NAPL. The r...

journal_title：Applied microbiology and biotechnology

authors： Birman I,Alexander M

更新日期：1996-03-01 00:00:00

abstract：:The microbial oxidation of ebastine to carebastine was investigated. Among the 15 micro-organisms examined, only the Cunninghamella strains showed the desired biotransformation. Cunninghamella blakesleeana oxidised the substrate within 7 days, via the intermediates alcohol and aldehyde, mainly to carebastine, the corr...

journal_title：Applied microbiology and biotechnology

authors： Schwartz H,Liebig-Weber A,Hochstätter H,Böttcher H

更新日期：1996-02-01 00:00:00

abstract：:The effect of the presence of supplementary glucose or acetate on the growth and pyridine-degrading activity of freely suspended and calcium-alginate-immobilized Pimelobacter sp. was investigated. Although the supplementary carbon sources could be degraded simultaneously with pyridine, Pimelobacter sp. exhibited a pre...

journal_title：Applied microbiology and biotechnology

authors： Rhee SK,Lee GM,Lee ST

更新日期：1996-02-01 00:00:00

abstract：:The organization of the genes of the penicillin cluster has been studied in three different mutants of P. chrysogenum impaired in penicillin biosynthesis. The three blocked mutants (derived from the parental strain P. chrysogenum Bb-1) lacked the genes pcbAB, pcbC and penDE of the penicillin biosynthetic pathway and w...

journal_title：Applied microbiology and biotechnology

authors： Fierro F,Montenegro E,Gutiérrez S,Martín JF

更新日期：1996-01-01 00:00:00

abstract：:A microbial peptide amidase was found in a limited screening and purified about 500-fold from Stenotrophomonas maltophilia. The native enzyme has a molecular mass of 38 kDa (gel filtration). The sequence of the first 16 amino acids was determined by Edman degradation. The isoelectric point was found to be around 5.8. ...

journal_title：Applied microbiology and biotechnology

authors： Stelkes-Ritter U,Wyzgol K,Kula MR

更新日期：1995-12-01 00:00:00

abstract：:A system for the controlled expression of a foreign gene in cultured tobacco cells (Nicotiana tabacum, BY2) by temperature shift was constructed. A 925-base-pair (bp) DNA fragment containing the 5'-flanking region of a low-molecular-mass heat-shock protein gene (HSP18.2) of Arabidopsis thaliana was inserted upstream o...

journal_title：Applied microbiology and biotechnology

authors： Yoshida K,Kasai T,Garcia MR,Sawada S,Shoji T,Shimizu S,Yamazaki K,Komeda Y,Shinmyo A

更新日期：1995-12-01 00:00:00

abstract：:Seven fractions rich in endoglucanase activity were separated from the extracellular cellulase system of the anaerobic rumen fungus Neocallimastix frontalis. The fractions (ES1, ES3, ES2U1, ES2U2, ES2U4, ES2U3C1 and ES2U3C2) were separated from each other and from a fraction that could solubilize crystalline cellulose...

journal_title：Applied microbiology and biotechnology

authors： Wood TM,Wilson CA,McCrae SI

更新日期：1995-12-01 00:00:00

abstract：:This study focused on the use the thermophilic anaerobic granulae in the start-up of 70 degrees C acetate-fed upflow anaerobic sludge-blanket (UASB) reactors and the kinetics of granulae grown at 70 degrees C. In the UASB reactors, chemical oxygen demand removal commenced within 48 h of the start-up. The maximum reduc...

journal_title：Applied microbiology and biotechnology

authors： Lepistö R,Rintala JA

更新日期：1995-11-01 00:00:00

abstract：:Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level alm...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Williamson G

更新日期：1995-11-01 00:00:00

abstract：:Plasmids without an origin of replication, but bearing the URA3 gene of Saccharomyces cerevisiae as a selective marker for transformation, are shown to replicate autonomously in Hansenula polymorpha, indicating that parts of the S. cerevisiae URA3 gene can fulfil an autonomous replication and stabilization function in...

journal_title：Applied microbiology and biotechnology

authors： Gatzke R,Weydemann U,Janowicz ZA,Hollenberg CP

更新日期：1995-10-01 00:00:00

abstract：:The utilization of monochlorobenzoate isomers (2-, 3- and 4-chlorobenzoate) by anaerobic microbial consortia in River Nile sediments was systematically evaluated under denitrifying, Fe-reducing, sulfidogenic and methanogenic conditions. Loss of all three chlorobenzoates was noted in denitrifying cultures; furthermore,...

journal_title：Applied microbiology and biotechnology

authors： Kazumi J,Häggblom MM,Young LY

更新日期：1995-10-01 00:00:00

abstract：:This review addresses metal uptake specifically by yeast. Metal uptake may be passive, active or both, depending on the viability of the biomass, and is influenced by a number of environmental and experimental factors. Uptake is typically accompanied by a degree of ion exchange and, under certain conditions, may be en...

journal_title：Applied microbiology and biotechnology

authors： Blackwell KJ,Singleton I,Tobin JM

更新日期：1995-08-01 00:00:00

abstract：:Nonadapted freshwater sediment slurries and sediment slurries adapted to dechlorinate 2,3-dichloropyridine (2,3-Cl2Pyd), 2,3-dichloroaniline (2,3-Cl2Anl), 2,3-dichlorophenol (2,3-Cl2PhOH), 3,5-dichloropyridine (3,5-Cl2Pyd), 3,5-dichloroaniline (3,5-Cl2Anl) and 3,5-dichlorophenol (3,5-Cl2PhOH) were studied to determine...

journal_title：Applied microbiology and biotechnology

authors： Liu SM,Jones WJ

更新日期：1995-08-01 00:00:00

abstract：:We have fused the epidermal growth factor (EGF) to the amino terminus of Pseudomonas exotoxin A (PE) to create a cytotoxic agent, designated EGF-PE, which preferentially kills EGF-receptor-bearing cells. In this study, we analyzed the effect of the Ia domain, the binding domain of PE on the cytotoxicity of EGF-PE towa...

journal_title：Applied microbiology and biotechnology

authors： Liao CW,Hseu TH,Hwang J

更新日期：1995-07-01 00:00:00

abstract：:Translational lacZ fusions to the promoters of the parasporal, crystal protein (binary toxin) and 100-kDa ADP-ribosylating mosquitocidal toxin genes of Bacillus sphaericus were prepared and expression of the toxin genes monitored as beta-galactosidase activity. Transcription of the crystal protein gene fusion began im...

journal_title：Applied microbiology and biotechnology

authors： Ahmed HK,Mitchell WJ,Priest FG

更新日期：1995-05-01 00:00:00